Structural Studies of Proteins by Mass Spectrometry

Targeted Quantitation of Proteins by Mass Spectrometry

Quantitative measurement of proteins is one of the most fundamental analytical tasks in a biochemistry laboratory, but widely used immunochemical methods often have limited specificity and high measurement variation. In this review, we discuss applications of multiple-reaction monitoring (MRM) mass spectrometry, which allows sensitive, precise quantitative analyses of peptides and the proteins ...

Tautomeric Equilibria Studies by Mass Spectrometry

Tautomerism in organic chemistry has been extensively studied in condensed phase by spectrometric methods, mainly by IR and NMR techniques. Mass spectrometry studies start 40 years ago but just recently it has been recognized the importance of the mass spectral data for the study of tautomerism in the gas phase. Mass spectrometry can provide valuable information in regard to tautomeric equilibr...

Secondary and Higher Order Structural Characterization of Peptides and Proteins by Mass Spectrometry

Insights into the activation of the helicase Prp43 by biochemical studies and structural mass spectrometry

Splicing of precursor messenger RNA is a hallmark of eukaryotic cells, which is carried out by the spliceosome, a multi-megadalton ribonucleoprotein machinery. The splicing reaction removes non-coding regions (introns) and ligates coding regions (exons). The spliceosome is a highly dynamic ribonucleoprotein complex that undergoes dramatic structural changes during its assembly, the catalysis an...

Intrinsic disorder in proteins: a challenge for (un)structural biology met by ion mobility-mass spectrometry.

The link between structure and function of a given protein is a principal tenet of biology. The established approach to understand the function of a protein is to 'solve' its structure and subsequently investigate interactions between the protein and its binding partners. However, structure determination via crystallography or NMR is challenging for proteins where localized regions or even thei...